Temperature-sensitive Escherichia coli mutant producing a temperature-sensitive sigma subunit of DNA-dependent RNA polymerase.

A gene affecting the sigma subunit of DNA-dependent RNA polymerase is tightly linked to dnaG at 66 min on the Escherichia coli chromosome. In order to create an easily selectable marker in this region, we inserted transposon-10, which carries a gene determining resistance to tetracycline (tet) near...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Harris, J D, Heilig, J S, Martinez, I I, Calendar, R, Isaksson, L A
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1978
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393142/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/366614/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.12.6177
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