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Temperature-sensitive Escherichia coli mutant producing a temperature-sensitive sigma subunit of DNA-dependent RNA polymerase.

A gene affecting the sigma subunit of DNA-dependent RNA polymerase is tightly linked to dnaG at 66 min on the Escherichia coli chromosome. In order to create an easily selectable marker in this region, we inserted transposon-10, which carries a gene determining resistance to tetracycline (tet) near...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Harris, J D, Heilig, J S, Martinez, I I, Calendar, R, Isaksson, L A
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1978
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393142/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/366614/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.12.6177
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