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Measuring Cytotoxicity by Bioluminescence Imaging Outperforms the Standard Chromium-51 Release Assay

The chromium-release assay developed in 1968 is still the most commonly used method to measure cytotoxicity by T cells and by natural killer cells. Target cells are loaded in vitro with radioactive chromium and lysis is determined by measuring chromium in the supernatant released by dying cells. Sin...

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Detalhes bibliográficos
Main Authors: Karimi, Mobin A., Lee, Eric, Bachmann, Michael H., Salicioni, Ana Maria, Behrens, Edward M., Kambayashi, Taku, Baldwin, Cynthia L.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3929704/
https://ncbi.nlm.nih.gov/pubmed/24586714
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0089357
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