An improved and robust DNA immunization method to develop antibodies against extra-cellular loops of multi-transmembrane proteins

Multi-transmembrane proteins are especially difficult targets for antibody generation largely due to the challenge of producing a protein that maintains its native conformation in the absence of a stabilizing membrane. Here, we describe an immunization strategy that successfully resulted in the iden...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Autori principali: Hazen, Meredith, Bhakta, Sunil, Vij, Rajesh, Randle, Steven, Kallop, Dara, Chiang, Vicki, Hötzel, Isidro, Jaiswal, Bijay S, Ervin, Karen E, Li, Bing, Weimer, Robby M, Polakis, Paul, Scheller, Richard H, Junutula, Jagath R, Hongo, Jo-Anne S
Natura: Artigo
Lingua:Inglês
Pubblicazione: Landes Bioscience 2014
Soggetti:
Report
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3929459/
https://ncbi.nlm.nih.gov/pubmed/24121517
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/mabs.26761
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !

Documenti analoghi