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To Nick or Not to Nick: Comparison of I-SceI Single- and Double-Strand Break-Induced Recombination in Yeast and Human Cells

Genetic modification of a chromosomal locus to replace an existing dysfunctional allele with a corrected sequence can be accomplished through targeted gene correction using the cell's homologous recombination (HR) machinery. Gene targeting is stimulated by generation of a DNA double-strand brea...

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Bibliografische gegevens
Hoofdauteurs: Katz, Samantha S., Gimble, Frederick S., Storici, Francesca
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science 2014
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3928301/
https://ncbi.nlm.nih.gov/pubmed/24558436
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0088840
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