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Using Single-Turnover Kinetics with Osmotic Stress to Characterize the EcoRV Cleavage Reaction
Type II restriction endonucleases require metal ions to specifically cleave DNA at canonical sites. Despite the wealth of structural and biochemical information, the number of Mg(2+) ions used for cleavage by EcoRV, in particular, at physiological divalent ion concentrations is still not established...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2013
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3923320/ https://ncbi.nlm.nih.gov/pubmed/24328115 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi401089y |
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