Absolute mRNA levels and transcriptional initiation rates in Saccharomyces cerevisiae.
We quantitate the absolute levels of individual mRNAs per yeast cell by hybridizing total yeast RNA with an excess of gene-specific 32P-oligonucleotides, and digesting the resulting RNA-DNA hybrids with S1 nuclease. By comparing the his3 hybridization signal from a known amount of yeast cells to the...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1996
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC39223/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8643554/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.11.5208 |
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