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Imaging the Glycosylation State of Cell Surface Glycoproteins using Two-photon Fluorescence Lifetime Imaging Microscopy

GLYCOPROTEINS IN FOCUS: Metabolic labeling of azido sugars combined with two-photon fluorescence lifetime imaging microscopy enables the visualization of specific glycoforms of endogenous proteins. This method can be utilized to detect glycosylated proteins in both cell culture and intact human tiss...

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Detalhes bibliográficos
Main Authors: Belardi, Brian, de la Zerda, Adam, Spiciarich, David R., Maund, Sophia L., Peehl, Donna M., Bertozzi, Carolyn R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3920747/
https://ncbi.nlm.nih.gov/pubmed/24259491
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201307512
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