Autoradiographic assay of mutants resistant to diphtheria toxin in mammalian cells in vitro.

Diphtheria toxin kills mammalian cells by ribosylating elongation factor 2, a protein factor necessary for protein synthesis. The frequency of cells able to form colonies in the presence of the toxin can be used as an assay for mutation to diphtheria toxin resistance. We report here that resistance...

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Main Authors: Ronen, A, Gingerich, J D, Duncan, A M, Heddle, J A
Format: Artigo
Jezik:Inglês
Izdano: 1984
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC391872/
https://ncbi.nlm.nih.gov/pubmed/6592605
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