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Autoradiographic assay of mutants resistant to diphtheria toxin in mammalian cells in vitro.

Diphtheria toxin kills mammalian cells by ribosylating elongation factor 2, a protein factor necessary for protein synthesis. The frequency of cells able to form colonies in the presence of the toxin can be used as an assay for mutation to diphtheria toxin resistance. We report here that resistance...

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發表在:Proc Natl Acad Sci U S A
Principais autores: Ronen, A, Gingerich, J D, Duncan, A M, Heddle, J A
格式: Artigo
語言:Inglês
出版: National Academy of Sciences 1984
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在線閱讀:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391872/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6592605/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.19.6124
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