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Activity-Based Diubiquitin Probes for Elucidating the Linkage Specificity of Deubiquitinating Enzymes
We report a new class of deubiquitinating enzyme (DUB) probes that resemble the native diubiquitin with a same linkage size and contain a Michael addition acceptor for trapping the DUB active-site cysteine. Both K63- and K48-linked diubiquitin probes were generated using a facile chemical ligation m...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3918478/ https://ncbi.nlm.nih.gov/pubmed/24225431 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c3cc47382a |
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