Isolation of an active transcription initiation complex from HeLa cell-free extract.
A two-step procedure has been developed for the formation of RNA polymerase II transcription initiation and elongation complexes. Initiation complexes are rapidly formed in HeLa cell-free extract supplemented with a DNA template containing the adenovirus 2 major late promoter and ATP. Assembly of tr...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1984
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391829/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6592595/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.19.5916 |
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