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Isolation of an active transcription initiation complex from HeLa cell-free extract.

A two-step procedure has been developed for the formation of RNA polymerase II transcription initiation and elongation complexes. Initiation complexes are rapidly formed in HeLa cell-free extract supplemented with a DNA template containing the adenovirus 2 major late promoter and ATP. Assembly of tr...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Tolunay, H E, Yang, L, Anderson, W F, Safer, B
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1984
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391829/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6592595/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.19.5916
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