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Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.

A new and highly effective method, termed suppression subtractive hybridization (SSH), has been developed for the generation of subtracted cDNA libraries. It is based primarily on a recently described technique called suppression PCR and combines normalization and subtraction in a single procedure....

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Diatchenko, L, Lau, Y F, Campbell, A P, Chenchik, A, Moqadam, F, Huang, B, Lukyanov, S, Lukyanov, K, Gurskaya, N, Sverdlov, E D, Siebert, P D
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC39182/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8650213/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.12.6025
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