RecA-Mediated Affinity Capture: A Method for Full-Length cDNA Cloning
We describe an improved method for rapid cloning of full-length cDNA from cDNA libraries. This approach is based on the ability of Escherichia coli RecA protein to form a stable nucleoprotein complex with a linear single-stranded DNA probe and homologous sequences in circular double-stranded DNA. Hy...
Na minha lista:
| Principais autores: | , , |
|---|---|
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis Group
1999-10-01
|
| coleção: | BioTechniques |
| Acesso em linha: | https://www.future-science.com/doi/10.2144/99274rr06 |
| Tags: |
Sem tags, seja o primeiro a adicionar uma tag!
|