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A degenerate PCR-based strategy as a means of identifying homologues of aminoglycoside and β-lactam resistance genes in the gut microbiota

BACKGROUND: The potential for the human gut microbiota to serve as a reservoir for antibiotic resistance genes has been the subject of recent discussion. However, this has yet to be investigated using a rapid PCR-based approach. In light of this, here we aim to determine if degenerate PCR primers ca...

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Autores principales: Fouhy, Fiona, Ross, R Paul, Fitzgerald, Gerald F, Stanton, Catherine, Cotter, Paul D
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2014
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3917905/
https://ncbi.nlm.nih.gov/pubmed/24499167
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-14-25
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