Restriction endonucleases can be used to study B-Z junctions in supercoiled DNA.
Plasmids containing (C-G)n inserts have been used to study the inhibition of cleavage by restriction endonucleases due to Z-DNA formation in negatively supercoiled plasmids. The enzyme BssHII, which recognizes G-C-G-C-G-C, is strongly inhibited when the insert forms Z-DNA. The BamHI recognition sequ...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1984
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391781/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6091104/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.18.5714 |
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