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Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum

Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable varian...

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Autores principales: Hentschel, Eva, Will, Cornelia, Mustafi, Nurije, Burkovski, Andreas, Rehm, Nadine, Frunzke, Julia
Formato: Artigo
Lenguaje:Inglês
Publicado: Blackwell Publishing Ltd 2013
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3917462/
https://ncbi.nlm.nih.gov/pubmed/22938655
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1751-7915.2012.00360.x
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