Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli.
The conditions necessary for high-level expression of methionyl bovine growth hormone (Met-bGH) in Escherichia coli were investigated. Plasmids were constructed that contain a thermoinducible runaway replicon and either the E. coli tryptophan or lipoprotein promoter and ribosome binding sites, which...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1984
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391712/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6089201/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.17.5403 |
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