Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli.

The conditions necessary for high-level expression of methionyl bovine growth hormone (Met-bGH) in Escherichia coli were investigated. Plasmids were constructed that contain a thermoinducible runaway replicon and either the E. coli tryptophan or lipoprotein promoter and ribosome binding sites, which...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Schoner, B E, Hsiung, H M, Belagaje, R M, Mayne, N G, Schoner, R G
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1984
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391712/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6089201/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.17.5403
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