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Expression of normal and transforming H-ras genes in Escherichia coli and purification of their encoded p21 proteins.

The H-ras gene of the BALB murine sarcoma virus (BALB-MSV) was placed under the transcriptional control of the tightly regulated PL promoter of bacteriophage lambda in the expression vectors pEV-vrf-1 and pRC23. Upon derepression of the PL promoter, large amounts (10-20% of total cellular protein) o...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Lacal, J C, Santos, E, Notario, V, Barbacid, M, Yamazaki, S, Kung, H, Seamans, C, McAndrew, S, Crowl, R
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1984
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391692/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6089191/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.17.5305
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