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Expression of normal and transforming H-ras genes in Escherichia coli and purification of their encoded p21 proteins.
The H-ras gene of the BALB murine sarcoma virus (BALB-MSV) was placed under the transcriptional control of the tightly regulated PL promoter of bacteriophage lambda in the expression vectors pEV-vrf-1 and pRC23. Upon derepression of the PL promoter, large amounts (10-20% of total cellular protein) o...
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| 出版年: | Proc Natl Acad Sci U S A |
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| 主要な著者: | , , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
1984
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391692/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6089191/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.17.5305 |
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