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A specific mutation abolishing Na+/H+ antiport activity in hamster fibroblasts precludes growth at neutral and acidic pH.

A H+-suicide technique based on the reversibility of Na+/H+ antiport was developed for the selection of mutants deficient in this membrane-bound activity. The strategy was to use the Na+/H+ antiporter as a H+-vector killing device. Chinese hamster lung fibroblasts (CCL39) were loaded with LiCl and i...

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Bibliografske podrobnosti
Main Authors: Pouysségur, J, Sardet, C, Franchi, A, L'Allemain, G, Paris, S
Format: Artigo
Jezik:Inglês
Izdano: 1984
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC391585/
https://ncbi.nlm.nih.gov/pubmed/6087349
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