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TnphoA: a transposon probe for protein export signals.

We constructed a derivative of transposon Tn5 that permits the generation of hybrid proteins composed of alkaline phosphatase (EC 3.1.3.1) lacking its signal peptide fused to amino-terminal sequences of other proteins. Such a hybrid gives alkaline phosphatase activity if the protein fused to alkalin...

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Bibliografische gegevens
Hoofdauteurs: Manoil, C, Beckwith, J
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1985
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC391456/
https://ncbi.nlm.nih.gov/pubmed/2999794
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