TnphoA: a transposon probe for protein export signals.
We constructed a derivative of transposon Tn5 that permits the generation of hybrid proteins composed of alkaline phosphatase (EC 3.1.3.1) lacking its signal peptide fused to amino-terminal sequences of other proteins. Such a hybrid gives alkaline phosphatase activity if the protein fused to alkalin...
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| Опубликовано в:: | Proc Natl Acad Sci U S A |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
National Academy of Sciences
1985
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391456/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2999794/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.23.8129 |
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