A rapid and sensitive method for detection and quantification of calcineurin and calmodulin-binding proteins using biotinylated calmodulin.

Purified bovine brain calmodulin was biotinylated with biotinyl-epsilon-aminocaproic acid N-hydroxysuccinimide. Biotinylated calmodulin was used to detect and quantify calmodulin-binding proteins following both protein blotting and slot-blot procedures by using alkaline phosphatase or peroxidase cou...

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Detalles Bibliográficos

Publicado en:	Proc Natl Acad Sci U S A
Autores principales:	Billingsley, M L, Pennypacker, K R, Hoover, C G, Brigati, D J, Kincaid, R L
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	National Academy of Sciences 1985
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC391377/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3865179/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.22.7585
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