A rapid and sensitive method for detection and quantification of calcineurin and calmodulin-binding proteins using biotinylated calmodulin.

Purified bovine brain calmodulin was biotinylated with biotinyl-epsilon-aminocaproic acid N-hydroxysuccinimide. Biotinylated calmodulin was used to detect and quantify calmodulin-binding proteins following both protein blotting and slot-blot procedures by using alkaline phosphatase or peroxidase cou...

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Autores principales: Billingsley, M L, Pennypacker, K R, Hoover, C G, Brigati, D J, Kincaid, R L
Formato: Artigo
Lenguaje:Inglês
Publicado: 1985
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Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC391377/
https://ncbi.nlm.nih.gov/pubmed/3865179
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