Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT)

The proper dissection of the molecular mechanisms governing the specification and differentiation of specific cell types requires isolation of pure cell populations from heterogeneous tissues and whole organisms. Here, we describe a method for purification of nuclei from defined cell or tissue types...

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Autors principals: Amin, Nirav M., Greco, Todd M., Kuchenbrod, Lauren M., Rigney, Maggie M., Chung, Mei-I, Wallingford, John B., Cristea, Ileana M., Conlon, Frank L.
Format: Artigo
Idioma:Inglês
Publicat: Company of Biologists 2014
Matèries:
Techniques and Resources
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3912835/
https://ncbi.nlm.nih.gov/pubmed/24496632
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.098327
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