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Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT)

The proper dissection of the molecular mechanisms governing the specification and differentiation of specific cell types requires isolation of pure cell populations from heterogeneous tissues and whole organisms. Here, we describe a method for purification of nuclei from defined cell or tissue types...

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Bibliografske podrobnosti
Main Authors: Amin, Nirav M., Greco, Todd M., Kuchenbrod, Lauren M., Rigney, Maggie M., Chung, Mei-I, Wallingford, John B., Cristea, Ileana M., Conlon, Frank L.
Format: Artigo
Jezik:Inglês
Izdano: Company of Biologists 2014
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3912835/
https://ncbi.nlm.nih.gov/pubmed/24496632
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.098327
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