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Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT)

The proper dissection of the molecular mechanisms governing the specification and differentiation of specific cell types requires isolation of pure cell populations from heterogeneous tissues and whole organisms. Here, we describe a method for purification of nuclei from defined cell or tissue types...

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Detalhes bibliográficos
Main Authors: Amin, Nirav M., Greco, Todd M., Kuchenbrod, Lauren M., Rigney, Maggie M., Chung, Mei-I, Wallingford, John B., Cristea, Ileana M., Conlon, Frank L.
Formato: Artigo
Idioma:Inglês
Publicado em: Company of Biologists 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3912835/
https://ncbi.nlm.nih.gov/pubmed/24496632
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.098327
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