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Identifying Zn-bound histidine residues in metalloproteins using hydrogen deuterium exchange mass spectrometry
In this work we have developed a method that uses hydrogen deuterium exchange (HDX) of C2-hydrogens of histidines coupled with mass spectrometry (MS) to identify Zn-bound histidines in metalloproteins. This method relies on differences in HDX reaction rates of Zn-bound and Zn-free His residues. Usin...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3912567/ https://ncbi.nlm.nih.gov/pubmed/24313328 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac4032719 |
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