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Identifying Zn-bound histidine residues in metalloproteins using hydrogen deuterium exchange mass spectrometry

In this work we have developed a method that uses hydrogen deuterium exchange (HDX) of C2-hydrogens of histidines coupled with mass spectrometry (MS) to identify Zn-bound histidines in metalloproteins. This method relies on differences in HDX reaction rates of Zn-bound and Zn-free His residues. Usin...

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Detalhes bibliográficos
Main Authors: Dong, Jia, Callahan, Katie L., Borotto, Nicholas B., Vachet, Richard W.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3912567/
https://ncbi.nlm.nih.gov/pubmed/24313328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac4032719
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