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Amplification and Purification of T4-Like Escherichia coli Phages for Phage Therapy: from Laboratory to Pilot Scale

We investigated the amplification and purification of phage preparations with respect to titer, contamination level, stability, and technical affordability. Using various production systems (wave bags, stirred-tank reactors, and Erlenmeyer flasks), we obtained peak titers of 10(9) to 10(10) PFU/ml f...

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Detalhes bibliográficos
Main Authors: Bourdin, Gilles, Schmitt, Bertrand, Marvin Guy, Laure, Germond, Jacques-Edouard, Zuber, Sophie, Michot, Lise, Reuteler, Gloria, Brüssow, Harald
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3911048/
https://ncbi.nlm.nih.gov/pubmed/24362424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03357-13
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