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Amplification and Purification of T4-Like Escherichia coli Phages for Phage Therapy: from Laboratory to Pilot Scale

We investigated the amplification and purification of phage preparations with respect to titer, contamination level, stability, and technical affordability. Using various production systems (wave bags, stirred-tank reactors, and Erlenmeyer flasks), we obtained peak titers of 10(9) to 10(10) PFU/ml f...

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Autores principales: Bourdin, Gilles, Schmitt, Bertrand, Marvin Guy, Laure, Germond, Jacques-Edouard, Zuber, Sophie, Michot, Lise, Reuteler, Gloria, Brüssow, Harald
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2014
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3911048/
https://ncbi.nlm.nih.gov/pubmed/24362424
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03357-13
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