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Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system

Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive...

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Bibliografiske detaljer
Main Authors: Zhao, Yicheng, Dai, Zhen, Liang, Yang, Yin, Ming, Ma, Kuiying, He, Mei, Ouyang, Hongsheng, Teng, Chun-Bo
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3909901/
https://ncbi.nlm.nih.gov/pubmed/24487629
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep03943
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