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Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system
Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3909901/ https://ncbi.nlm.nih.gov/pubmed/24487629 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep03943 |
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