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Chemical Rescue and Inhibition Studies to Determine the Role of Arg301 in Phosphite Dehydrogenase

Phosphite dehydrogenase (PTDH) catalyzes the NAD(+)-dependent oxidation of phosphite to phosphate. This reaction requires the deprotonation of a water nucleophile for attack on phosphite. A crystal structure was recently solved that identified Arg301 as a potential base given its proximity and orien...

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Detalhes bibliográficos
Main Authors: Hung, John E., Fogle, Emily J., Garg, Neha, Chekan, Jonathan R., Nair, Satish K., van der Donk, Wilfred A.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3909101/
https://ncbi.nlm.nih.gov/pubmed/24498026
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0087134
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