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Exposure of actin thiols by the removal of tightly held calcium ions.

The removal of bound metal ions from G-actin uncovered two thiols, Cys-10 and Cys-257. The uncovering of these thiols requires a free calcium concentration lower than 10 nM. Therefore, participation of one or both thiols in Ca2+ binding is suggested. Actin labeled with N-(5-fluoresceinyl)maleimide i...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Konno, K, Morales, M F
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 1985
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390878/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3865205/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.23.7904
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