Exposure of actin thiols by the removal of tightly held calcium ions.
The removal of bound metal ions from G-actin uncovered two thiols, Cys-10 and Cys-257. The uncovering of these thiols requires a free calcium concentration lower than 10 nM. Therefore, participation of one or both thiols in Ca2+ binding is suggested. Actin labeled with N-(5-fluoresceinyl)maleimide i...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1985
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390878/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3865205/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.23.7904 |
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