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Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli.
A cDNA library was constructed from firefly (Photinus pyralis) lantern poly(A)+ RNA, using the Escherichia coli expression vector lambda gt11. The library was screened with anti-P. pyralis luciferase (Photinus luciferin:oxygen 4-oxidoreductase, EC 1.13.12.7) antibody, and several cDNA clones express...
Wedi'i Gadw mewn:
| Cyhoeddwyd yn: | Proc Natl Acad Sci U S A |
|---|---|
| Prif Awduron: | , , , |
| Fformat: | Artigo |
| Iaith: | Inglês |
| Cyhoeddwyd: |
National Academy of Sciences
1985
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| Pynciau: | |
| Mynediad Ar-lein: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390871/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3906652/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.23.7870 |
| Tagiau: |
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