Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli.

A cDNA library was constructed from firefly (Photinus pyralis) lantern poly(A)+ RNA, using the Escherichia coli expression vector lambda gt11. The library was screened with anti-P. pyralis luciferase (Photinus luciferin:oxygen 4-oxidoreductase, EC 1.13.12.7) antibody, and several cDNA clones express...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	de Wet, J R, Wood, K V, Helinski, D R, DeLuca, M
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1985
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390871/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3906652/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.23.7870
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