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Mapping the cysteine residues and actin-binding regions of villin by using antisera to the amino and carboxyl termini of the molecule.

Peptide antisera specific for either the amino- or carboxyl-terminal regions of villin were used to locate the position of cysteine residues in immunoblots of villin cleaved with 2-nitro-5-thiocyanobenzoic acid. Maps constructed from the cleavage pattern suggest that villin contains six cysteine res...

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Detalhes bibliográficos
Main Authors: Matsudaira, P, Jakes, R, Cameron, L, Atherton, E
Formato: Artigo
Idioma:Inglês
Publicado em: 1985
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC390772/
https://ncbi.nlm.nih.gov/pubmed/2413446
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