Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene.

We describe a procedure for detecting high-affinity, sequence-specific DNA-binding proteins from crude nuclear extracts. The technique utilizes electrophoretic transfer of NaDodSO4/PAGE-fractionated proteins onto nitrocellulose filters. Incubation of the filters with a 5% (wt/vol) solution of nonfat...

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Hauptverfasser: Miskimins, W K, Roberts, M P, McClelland, A, Ruddle, F H
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1985
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC390762/
https://ncbi.nlm.nih.gov/pubmed/2995982
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