Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene.

We describe a procedure for detecting high-affinity, sequence-specific DNA-binding proteins from crude nuclear extracts. The technique utilizes electrophoretic transfer of NaDodSO4/PAGE-fractionated proteins onto nitrocellulose filters. Incubation of the filters with a 5% (wt/vol) solution of nonfat...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Miskimins, W K, Roberts, M P, McClelland, A, Ruddle, F H
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1985
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390762/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2995982/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.20.6741
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