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Generation of Human Induced Pluripotent Stem (iPS) Cells in Serum- and Feeder-Free Defined Culture and TGF-β1 Regulation of Pluripotency

Human Embryonic Stem cells (hESCs) and human induced Pluripotent Stem cells (hiPSCs) are commonly maintained on inactivated mouse embryonic fibroblast as feeder cells in medium supplemented with FBS or proprietary replacements. Use of culture medium containing undefined or unknown components has lim...

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Detalhes bibliográficos
Main Authors: Yamasaki, Sachiko, Taguchi, Yuki, Shimamoto, Akira, Mukasa, Hanae, Tahara, Hidetoshi, Okamoto, Tetsuji
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3906124/
https://ncbi.nlm.nih.gov/pubmed/24489856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0087151
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