Expression of enzymatically active reverse transcriptase in Escherichia coli.
Reverse transcriptase of murine retroviruses is a monomeric protein of approximately 80,000 daltons, which is encoded by the central portion of the viral pol gene. To prepare large quantities of the enzyme, we have constructed gene fusions between the trpE gene and portions of the pol gene of Molone...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1985
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390474/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2410910/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.15.4944 |
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