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Expression of enzymatically active reverse transcriptase in Escherichia coli.

Reverse transcriptase of murine retroviruses is a monomeric protein of approximately 80,000 daltons, which is encoded by the central portion of the viral pol gene. To prepare large quantities of the enzyme, we have constructed gene fusions between the trpE gene and portions of the pol gene of Molone...

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Bibliografische gegevens
Gepubliceerd in:Proc Natl Acad Sci U S A
Hoofdauteurs: Tanese, N, Roth, M, Goff, S P
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: National Academy of Sciences 1985
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Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390474/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2410910/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.15.4944
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