Quantification of Signaling Lipids by Nano-Electrospray Ionization Tandem Mass Spectrometry (Nano-ESI MS/MS)

Lipids, such as phosphoinositides (PIPs) and diacylglycerol (DAG), are important signaling intermediates involved in cellular processes such as T cell receptor (TCR)-mediated signal transduction. Here we report identification and quantification of PIP, PIP(2) and DAG from crude lipid extracts. Capit...

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Autores principales: Haag, Mathias, Schmidt, Angelika, Sachsenheimer, Timo, Brügger, Britta
Formato: Artigo
Lenguaje:Inglês
Publicado: MDPI 2012
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3901191/
https://ncbi.nlm.nih.gov/pubmed/24957368
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/metabo2010057
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