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Single-molecule analysis of gene expression using two-color RNA labeling in live yeast
Live-cell imaging of mRNA yields important insights into gene expression, but it has generally been limited to the labeling of one RNA species and has never been used to count single mRNAs over time in yeast. We demonstrate a two-color imaging system with single-molecule resolution using MS2 and PP7...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3899799/ https://ncbi.nlm.nih.gov/pubmed/23263691 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2305 |
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