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Nanopore-based identification of individual nucleotides for direct RNA sequencing

We describe a label-free ribobase identification method, which uses ionic current measurement to resolve ribonucleoside monophosphates or diphosphates in α-hemolysin protein nanopores containing amino-cyclodextrin adapters. The accuracy of base identification is further investigated through the use...

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Detalhes bibliográficos
Main Authors: Ayub, Mariam, Hardwick, Steven W., Luisi, Ben F., Bayley, Hagan
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3899427/
https://ncbi.nlm.nih.gov/pubmed/24171554
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/nl403469r
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