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Altered Sequences Changing the Operator-Binding Properties of the Lac Repressor: Colinearity of the Repressor Protein with the i-Gene Map

A technique is described for mapping point mutations in the first 59 amino-acid residues of the lac repressor from Escherichia coli, using less than 0.1 μmol (4 mg) of the purified protein. This technique was used to localize five mutations affecting the ability of the i-gene product to repress in v...

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書誌詳細
出版年:Proc Natl Acad Sci U S A
主要な著者: Weber, Klaus, Platt, Terry, Ganem, Don, Miller, Jeffrey H.
フォーマット: Artigo
言語:Inglês
出版事項: National Academy of Sciences 1972
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC389835/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4566452/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.69.12.3624
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