Altered Sequences Changing the Operator-Binding Properties of the Lac Repressor: Colinearity of the Repressor Protein with the i-Gene Map
A technique is described for mapping point mutations in the first 59 amino-acid residues of the lac repressor from Escherichia coli, using less than 0.1 μmol (4 mg) of the purified protein. This technique was used to localize five mutations affecting the ability of the i-gene product to repress in v...
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| Опубликовано в:: | Proc Natl Acad Sci U S A |
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| Главные авторы: | , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
National Academy of Sciences
1972
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC389835/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4566452/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.69.12.3624 |
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